Aria 8.0 Podręcznik Użytkownika Pobierz pdf (Strona 63)

Chapter 2: Theory of Operation 63

Note that changes to values in the Sort Setup windows (Breakoff and Side

Stream) are automatically saved. At startup, the last settings used on the

cytometer are restored, except the Stream and Sweet Spot controls which always

default to off. For more information, see Sort Setup on page 101.

Table 2-1 Breakoff window controls

Control Description

Stream button Turns the stream on and off.

Sweet Spot

button

Enables automatic adjustment of the drop drive

amplitude to maintain the stability of the breakoff

point.

When the Sweet Spot is on, the Amplitude and

Frequency fields are disabled. The amplitude is

automatically adjusted by the software. To enable the

fields, turn off the Sweet Spot.

Amplitude field Adjusts the amplitude or intensity of the drop drive,

from 1.0–80.0 volts.

The drop drive amplitude determines the breakoff

point. A higher amplitude value results in a shorter

stream breakoff. A lower amplitude results in a longer

stream breakoff. Typically, the amplitude is set once, at

the beginning of a sorting experiment, and then

maintained via the Sweet Spot.

Frequency field Determines the number of drops formed per second and

the size of the drops. (Drop size is also influenced by

the nozzle size.)

The drop drive frequency can be adjusted from 1.0–

102.0 kHz. The higher the frequency, the more drops

are generated per second and the smaller the drops. The

lower the frequency, the fewer drops generated per

second and the larger the drops.

off

on off

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Aria 8.0 Podręcznik Użytkownika Strona 63