Aria 8.0 Podręcznik Użytkownika Pobierz pdf (Strona 166)

166 BD FACSAria II User’s Guide

If you cannot see a stream image or the image is dim, adjust the micrometer

dial on the diode laser to better view the streams (Figure 1-20 on page 38).

5 Adjust the 2nd, 3rd, and 4th Drop settings to tighten the center stream and

fine-tune the side streams, if needed.

Generally, the Sort Setup mode provides good starting values for these

settings. Adjust the values only if needed to optimize the streams.

6 Open the aspirator drawer and aim the side stream(s) into each collection

tube.

a In the Side Stream window, click the Waste Drawer button ( ) to

open the drawer.

b Open the sort block door and aim each side stream into the tube as you

adjust the corresponding slider in the Side Stream window.

c When you are satisfied with the side stream deflection, close the sort

block door.

7 Click the Voltage button ( ) to turn off the deflection plates.

Determining the Drop Delay – Manual Method

BD FACS Accudrop technology is used to determine the optimal drop delay

setting for your sorting application. For more information, see Drop Delay

Overview on page 67.

There are two methods for determining the drop delay.

• Manual drop delay – using the standard method, as described in the

following sections.

•Auto drop delay – using an automated algorithm method. See Determining

the Drop Delay – Automatic Method on page 171.

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Aria 8.0 Podręcznik Użytkownika Strona 166