Aria 8.0 Podręcznik Użytkownika Pobierz pdf (Strona 59)

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Chapter 2: Theory of Operation 59

Laser Delay

Sample interrogation takes place within the cuvette flow cell. Fiber optic cables

are used to direct laser light through a series of prisms that focus each laser on

the stream at a separate position. This allows optimal detection of fluorescence

signals from each laser with minimal cross-contamination from the other beams.

In a three-laser system, the red laser intercepts the stream first, followed by the

blue and then the violet laser. Because the laser signals are spatially separated,

there is a slight delay between the detection of each laser’s signal (Figure 2-13).

Figure 2-13 Signal separation over time

The delay factor in BD FACSDiva software is used to realign the signals so they

can be measured and displayed on the same time scale. Note that signals are

aligned with respect to the blue laser, so the red laser signals always have a

negative delay value.

time

cell enters and leaves

the first laser intercept

cell enters and leaves the

second laser intercept

cell enters and leaves

the third laser intercept

red

blue

violet

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