Aria 8.0 Podręcznik Użytkownika Pobierz pdf (Strona 122)

122 BD FACSAria II User’s Guide

Setting Up the Fluidics Cart

Check the fluid levels in the sheath tank and waste containers every time you use

the cytometer. This ensures that you will not run out of sheath fluid during an

experiment or have to service the containers during a sort. Fluidics level

indicators are shown in the Cytometer window in BD FACSDiva software. See

Figure 4-2 on page 115.

NOTE When the fluidics cart is in the correct position in the lab, lock the rolling

casters by turning the orange disk until the feet contact the floor. This helps

minimize any vibrations that could affect the stability of the stream.

If a fluidics container is running low, refill it with the fluid indicated on the

container label. For best results, fill the containers only with the fluids shown in

Table 4-2. For ordering information, see Reagents on page 268.

For instructions on emptying the waste, see page 127.

Table 4-2 Recommended fluids

Container Compatible Fluids Capacity

Sheath

a. Users should select solutions that are compatible with their specific samples and experiments.

• BD FACSFlow™ sheath fluid (non-sterile)

• 1X phosphate-buffered saline (PBS), with or without

preservatives

one 10-L

stainless steel

container

Ethanol

Shutdown

70% solution diluted in DI or laboratory-grade water one 5-L stainless

steel container

Bleach • 10% household bleach (0.5% sodium hypochlorite)

•BD™ FACSClean solution

5 L

DI Deionized water (Milli-Q or equivalent). Add 3 mL of

bleach per liter of DI water.

5 L

Ethanol 70% solution diluted in DI or laboratory-grade water 5 L

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Aria 8.0 Podręcznik Użytkownika Strona 122