Aria 8.0 Podręcznik Użytkownika Pobierz pdf (Strona 35)

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Chapter 1: Cytometer Components 35

Collection Optics

From the cuvette flow cell, laser light is collected by a fluorescence objective lens

that is gel-coupled to the cuvette to transmit the maximum amount of light. The

lens collects and focuses fluorescent light emitted at each of the laser focal points

onto individual collection fibers. These fibers transfer the emitted light to the

collection optics, as shown in Figure 1-17.

Figure 1-17 Side view of flow cell, nozzle, and objective lens

The collection optics are set up in octagon- and trigon-shaped arrays that are

engineered to maximize signal detection from each laser. This is accomplished by

transmitting the highest wavelengths to the first photomultiplier tube (PMT), and

reflecting lower wavelengths to the next PMT through a series of longpass

dichroic mirrors. Bandpass filters in front of each PMT allow fine-tuning of the

spectral wavelengths that need to be collected. Since reflection is more efficient

than transmittance, this design greatly increases the multicolor detection

capabilities of the cytometer. See Figure 1-18 on page 36.

nozzle

cuvette flow cell

stream

fluorescence objective lens

individual collection

fibers

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