Aria 8.0 Podręcznik Użytkownika Pobierz pdf (Strona 171)

Chapter 5: Sorting 171

Determining the Drop Delay – Automatic Method

The Auto Drop Delay feature automates setting the drop delay to get optimized

results from sorting. You should use this method after you have a good

understanding of how drop delay works in general. See Using Manual Drop

Delay on page 168 for more information.

Overview of Auto Drop Delay

Auto Drop Delay works best when the sort system is stable and assumes that BD

Accudrop beads are used. When the process is started, the auto drop algorithm

uses several passes to find best drop delay possible under current conditions of

the system. Coarse passes are used to find initial drop delay within 2 drops of the

ideal. The coarse passes are faster than fine tune passes. Fine tune passes are used

to locate the drop delay value which is ideal considering current conditions.

Using Auto Drop Delay

1 Set up an experiment for drop delay as described in Setting Up the

Experiment on page 167.

2 Load a tube filled with a suspension of BD Accudrop beads (approximately

2 drops of beads in 0.5 mL PBS).

3 Adjust the flow rate to achieve these values of events per second:

70 micron = 1,000 to 3,000

85 micron = 800 to 2,000

100 micron = 600 to 1,500

130 micron = 400 to 1,200

; Tip If this cannot be achieved using a flow rate setting between 1 and 5,

adjust the bead concentration.

4 Turn on the voltage in the Side Stream window. Click Sort in the Sort

Layout window.

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Aria 8.0 Podręcznik Użytkownika Strona 171