Aria 8.0 podręczniki

Aria 8.0 Instrukcja Użytkownika (16 strony)

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Angielski

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Flow Cytometry Core Facility

1

Prepared by Marc Barnard

1

Table of Contents

1

Introduction

2

Starting up the system

4

Optimizing the side streams

6

Aiming the streams

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Page 10

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Page 11

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Page 12

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Page 13

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Page 14

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Page 15

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Aria 8.0 Instrukcja Użytkownika (23 strony)

Marka: Aria | Kategoria: Tablety | Rozmiar: 1.02 MB |

Rumuński

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Manual de utilizare

1

Evolio Aria 8.0

1

Măsuri de protecţie

2

Cuprins

5

Accesorii

6

Butoane şi porturi

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Încărcarea bateriei

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Pornirea unităţii

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Ecranul de start

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Aplicaţii preinstalate

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Deblocarea ecranului

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Accesul la Internet

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Lansarea browserului de Web

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Biblioteca de fişiere audio

12

Vizualizarea fotografiilor

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Navigarea în fişiere

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Setarea contului de email

18

Gestionarea conturilor

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Ieşirea HDMI

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Gestionarea operaţiunilor

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Depanare

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Specificaţii

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Aria 8.0 Podręcznik Użytkownika (344 strony)

Marka: Aria | Kategoria: Tablety | Rozmiar: 11.23 MB |

Angielski

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BD FACSAria II

1

User’s Guide

1

FCC Information

3

Contents

5

About This Guide

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Conventions

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Technical Assistance

15

Limitations

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Cytometer Components

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Fluidics Cart

18

Power and Operation

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Flow Cytometer

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Fluidics Components

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Sample Injection Chamber

25

Tube Holders

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Cuvette Flow Cell

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Figure 1-11 Nozzle

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Sort Block

29

Deflection Plates

30

Aspirator Drawer

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Aerosol Management

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Sort Collection Chamber

32

Optics System

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Collection Optics

35

Detectors

36

Stream-Viewing Optics

38

Cytometer Electronics

39

Emergency Stop Button

40

Workstation

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Theory of Operation

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Fluid Movement

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Sample Flow

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Hydrodynamic Focusing

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Signal Generation

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Fluorescent Signals

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Signal Detection

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Longpass Filters

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Bandpass Filters

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% transmission

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BP 500/50 filter

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DF 500/50 filter

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Neutral Density Filters

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Electronic Processing

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Pulse Parameters

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Laser Delay

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Drop Formation

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Breakoff Window

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Side Stream Formation

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Drop Delay Overview

67

Drop Charging

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Yield Mask

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Purity Mask

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Phase Mask

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Sort Precision Modes

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Defining New Precision Modes

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Using BD FACSDiva Software

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Workspace Components

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Cytometer Controls

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Change Sample Filter

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Cleaning Modes

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Sheath Pressure

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Sample Agitation

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Sample Temperature

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Fluidics Level Indicators

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Cytometer Configuration

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Cytometer Status Report

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Custom Configurations

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Copying a Base Configuration

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Select the Sort Setup that

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Configuration Mismatch Dialog

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Acquisition Controls

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Sorting Controls

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Sort Menu

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Sort Setup

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Sort Layout

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Setting Up a Sort Layout

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Editing a Sort Layout

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Using Sorting Controls

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Using Counters

108

Monitoring a Sort

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Sort Report

110

Templates

112

Running Samples

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Cytometer Startup

114

Performing Fluidics Startup

115

Starting the Stream

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Setting Up the Breakoff

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Setting Up the Fluidics Cart

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Refilling the Sheath Tank

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ATTENTION

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Emptying the Waste Container

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Waste (A)

129

Preparing the CS&T Beads

133

Running a Performance Check

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Reviewing the Results

134

Application Settings

136

Adjusting Area Scaling

137

Figure 4-14 FSC and SSC Plot

138

Optimizing PMT Voltages

141

Saving Application Settings

143

Data Collection

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Calculating Compensation

148

Data Recording and Analysis

151

Setting Up the Experiment

152

Recording Data

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Analyzing Data

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Performing a Batch Analysis

159

Setting Up for Sorting

162

Setting Up for Bulk Sorting

164

Using Manual Drop Delay

168

Overview of Auto Drop Delay

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Using Auto Drop Delay

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Pausing and Resuming a Sort

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Setting Up the Stream

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Test Sort button

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Creating a Custom Device

184

Deleting a Custom Device

186

Shutdown and Maintenance

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Daily Shutdown

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Fluidics Shutdown

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External Cleaning

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Scheduled Maintenance

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Sample Line Backflush

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Prime After Tank Refill

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Prepare for Aseptic Sort

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DI water port

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DI water line

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Purging the Fluid Filters

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Purging the Sheath Filter

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Changing Fluid Filters

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Changing the Sheath Filter

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Changing the Sample Lines

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Figure 6-12 Ferrule tool

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Pinch Valve End

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Flow Cell End

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Changing the Air Filters

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Unscheduled Maintenance

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Changing Nozzles

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Cleaning the Nozzle

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Cleaning the Camera Windows

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Upper Camera Window

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Apply lubricant to outside

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Using Custom Optical Filters

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Cleaning the Optical Filters

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FSC ND filter

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Set screw

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Nozzle holder

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Troubleshooting

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Troubleshooting the Stream

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Troubleshooting the Breakoff

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Sorting Troubleshooting

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Acquisition Troubleshooting

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Fluidics Troubleshooting

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Electronics Troubleshooting

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Technical Specifications

253

Cytometer Specifications

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Performance

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Sort Performance

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Excitation Optics

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Emission Optics

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Detector Arrays

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Fluidics Cart Specifications

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Appendix A

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Supplies and Consumables

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Cytometer Supplies

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Accessory Kit

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Other Replacement Parts

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Consumables

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Reagents

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Appendix B

271

Near UV Laser Option

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System Laser Configurations

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Specifications

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Operation

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Instrument Setup

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Experiment Setup Tips

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Appendix C

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BD Aerosol Management Option

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Option Components

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ULPA Filter

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Starting Up the Evacuator

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Maintenance

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Replacing the ULPA Filter

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Reset button

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Replacing the Air Filter

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Appendix D

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Temperature Control Option

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Setting Up the Water Bath

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Cooling OutCooling In

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Setting Up the Tube Holder

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Input tubing

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Output tubing

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Setting Up the ACDU Stage

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Starting Up the Water Bath

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Appendix E

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QC Using BD FACSDiva Software

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Software

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Preparing QC Particles

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Setting Up the QC Experiment

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Recording QC Data

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Figure E-6 Recorded QC data

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Reusing the QC Experiment

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Tracking QC Results

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Numerics

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